Insemination of oocytes

 

Oocyte inseminated via conventional IVF
Oocyte inseminated via conventional IVF

Oocytes are inseminated between 4-6 hours after harvesting.

There are two techniques: conventional insemination (IVF) and intracytoplasmic sperm injection (ICSI).

Conventional insemination

For conventional insemination, the mobile sperm concentration obtained following semen processing must be adjusted to about 100,000 per oocyte. The technique consists in depositing the sperm in a culture dish together with the mature oocytes. The labelled dishes are stored at 37º under appropriate humidity and gaseous conditions until the following day when the embryologist will check if fertilisation has occurred or not.

Intracytoplasmic sperm injection (ICSI) Intracytoplasmic sperm injection (ICSI) Intracytoplasmic sperm injection (ICSI)
Intracytoplasmic sperm injection (ICSI)

Intracytoplasmic sperm injection (ICSI)

This technique involves the injection of a single sperm into each oocyte.

ICSI is used when male infertility factors cannot be resolved with conventional IVF. The use of this technique is determined by the gynaecologist, andrologist or embryologist, depending on the characteristics of the sperm. It is also indicated in cases of unexplained infertility or in cases of previous fertilisation failures.

Between 2-4 hours after oocyte retrieval, the embryologist removes the follicular cells surrounding the oocyte in order to observe the oocyte in greater detail. The oocytes are classified according to their maturity and are stored in culture dishes in an incubator. Only mature oocytes are microinjected.

Once the process is complete, the oocytes are stored in suitably labelled culture dishes and stored at 37º under appropriate humidity and gaseous conditions until the following day, when the embryologist will check if fertilisation has occurred or not.

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